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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal ligadura e punção induzida Sepsis como um modelo para estudar a autofagia em Ratos
 
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Cecal ligadura e punção induzida Sepsis como um modelo para estudar a autofagia em Ratos

Article doi: 10.3791/51066
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

Summary
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February 9th, 2014

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Sepse experimental pode ser induzida em camundongos usando a ligadura e punção do ceco método (CLP). Protocolos correntes para avaliar a autofagia in vivo no contexto de sepsia induzida por CLP são aqui apresentados: Um protocolo para medir autofagia utilizando ratinhos (GFP)-LC3, e um protocolo para medir a formação de autophagosome por microscopia electrónica.

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