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Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal ligatur og Punktering-induceret sepsis som en model til at studere Autophagy i mus
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Cecal ligatur og Punktering-induceret sepsis som en model til at studere Autophagy i mus

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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Eksperimentel sepsis kan induceres i mus ved hjælp af den cecal ligatur og punktering (CLP) metode. Nuværende protokoller for at vurdere autofagi in vivo i forbindelse med CLP-induceret sepsis præsenteres her: En protokol til måling autofagi anvendelse af (GFP)-LC3 mus og en protokol til måling af autophagosome dannelse ved elektronmikroskopi.

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Infektion autophagosome Autophagy cecal ligatur og punktering mus sepsis
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