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Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal ligature et ponction induite Sepsis comme un modèle pour étudier autophagie chez les souris
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Cecal ligature et ponction induite Sepsis comme un modèle pour étudier autophagie chez les souris

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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La septicémie expérimentale peut être induite chez des souris en utilisant la méthode (CLP) ligature caecale et ponction. Les protocoles actuels pour évaluer autophagy in vivo dans le cadre de la sepsie induite par CLP sont présentés ici: Un protocole pour la mesure en utilisant des souris autophagy (GFP)-LC3, et d'un protocole de mesure de la formation de autophagosome par microscopie électronique.

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Infection autophagosome autophagie la ligature du caecum et à la perforation la souris la septicémie
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