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Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal ligadura e punção induzida Sepsis como um modelo para estudar a autofagia em Ratos
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Cecal ligadura e punção induzida Sepsis como um modelo para estudar a autofagia em Ratos

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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Sepse experimental pode ser induzida em camundongos usando a ligadura e punção do ceco método (CLP). Protocolos correntes para avaliar a autofagia in vivo no contexto de sepsia induzida por CLP são aqui apresentados: Um protocolo para medir autofagia utilizando ratinhos (GFP)-LC3, e um protocolo para medir a formação de autophagosome por microscopia electrónica.

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Infecção autophagosome autofagia ligadura e punção do ceco ratos sepse
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