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Journal / Immunology and Infection / Çekal ligasyon ve Farelerde Autophagy incelemek…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Çekal ligasyon ve Farelerde Autophagy incelemek için bir model olarak Sepsis Patamaya kaynaklı
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Çekal ligasyon ve Farelerde Autophagy incelemek için bir model olarak Sepsis Patamaya kaynaklı

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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Deneysel sepsis çekal ligasyon ve delme (CLP) yöntemini kullanarak farelerde neden olabilir. CLP kaynaklı sepsis bağlamında in vivo Otofaji değerlendirmek için mevcut protokoller burada sunulmuştur: (GFP)-LC3 fareler kullanılarak Otofaji ölçülmesi için bir protokol ve elektron mikroskobu ile autophagosome oluşumunu ölçmek için bir protokolü.

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Enfeksiyon Sayı 84 autophagosome Autophagy çekum ligasyonu ve delinme fareler sepsis
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