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Immunology and Infection

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JoVE Journal Immunology and Infection
Ekspression af funktionelle Rekombinant hæmagglutinin og neuraminidase Proteiner fra Novel H7N9 influenza virus ved hjælp baculovirusekspressionssystemet
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Journal (Immunology and Infection)
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Detection of True IgE-expressing Mouse B Lineage Cells
Journal (Immunology and Infection)
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Ekspression af funktionelle Rekombinant hæmagglutinin og neuraminidase Proteiner fra Novel H7N9 influenza virus ved hjælp baculovirusekspressionssystemet

Article DOI: 10.3791/51112-v • 12:18 min • November 6th, 2013
November 6th, 2013 •
1,2, 1,3, 1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biological Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Medicine, Icahn School of Medicine at Mount Sinai

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Her beskriver vi en måde at udtrykke foldede og funktionelle korrekt influenzavirus overfladeantigener afledt fra romanen kinesiske H7N9 virus i insektceller. Teknikken kan tilpasses til at udtrykke ektodomæner eventuelle virale eller cellulære overfladeproteiner.

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