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Dissektion af
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
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Lineage-Specific Cytochemical Staining of Mesenchymal Stem Cells for Characterizing Differentiated Cells (Video) | JoVE
Journal (Biology)
Lineage-Specific Cytochemical Staining of Mesenchymal Stem Cells for Characterizing Differentiated Cells (Video) | JoVE
 
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Dissektion af

Article DOI: 10.3791/51118-v • 09:07 min • March 4th, 2014
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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Denne protokol beskriver, hvordan man dissekere premigratory kraniel neuralkam (NC) fra Xenopus laevis neurulas. Disse eksplantater kan udpladet på fibronectin og dyrket in vitro, eller podes tilbage i værten embryoner. Denne teknik gør det muligt at studere de mekanismer NC epitel-til-mesenchyme overgang, migration og differentiering.

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Developmental Biology neuralkam graft fibronectin
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