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JoVE Journal Biology
Dissection de
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
Journal (Biology)
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
Journal (Biology)
Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
 
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Dissection de

Article DOI: 10.3791/51118-v • 09:07 min • March 4th, 2014
March 4th, 2014 •
1,2,3,4, 1,2,3,4
1Institut Curie, Centre Universitaire, 2Université Paris Sud, Centre Universitaire, 3CNRS UMR 3347, Centre Universitaire, 4INSERM U1021, Centre Universitaire

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Ce protocole décrit comment disséquer la crête neurale crânienne prémigratoire (NC) de Xenopus laevis neurulas. Ces explants peuvent être étalées sur la fibronectine et cultivées in vitro, ou greffés retour dans des embryons hôtes. Cette technique permet d'étudier les mécanismes de la NC transition épithélium-à-mésenchyme, la migration et la différenciation.

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Biologie du Développement numéro 85 crête neurale, Embryon la dissection la greffe la fibronectine
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