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JoVE Journal
Immunology and Infection

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生发中心的高度已解决活体条纹照明显微镜
 
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生发中心的高度已解决活体条纹照明显微镜

Article DOI: 10.3791/51135-v 10:07 min April 9th, 2014
April 9th, 2014

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Summary

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Click here for the English version.

高分辨率的活体成像具有增强对比度高达120微米的深度在成年小鼠的淋巴结被空间调制多焦点双光子显微镜的激励模式来实现。在100微米的深度,我们测量了487 nm的分辨率(横向)和551纳米(轴向),从而绕过散射和衍射极限。

Tags

免疫学,第86期,双光子激光扫描显微术,深层组织活体成像,生发中心,淋巴结,高清晰度,对比度提高
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