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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
生发中心的高度已解决活体条纹照明显微镜
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
Journal (Immunology and Infection)
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
Journal (Immunology and Infection)
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
 
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生发中心的高度已解决活体条纹照明显微镜

Article DOI: 10.3791/51135
April 9th, 2014 •
*1,2, *3, 4, 2, *3,5, *1
1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
* These authors contributed equally

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April 9th, 2014

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高分辨率的活体成像具有增强对比度高达120微米的深度在成年小鼠的淋巴结被空间调制多焦点双光子显微镜的激励模式来实现。在100微米的深度，我们测量了487 nm的分辨率（横向）和551纳米（轴向），从而绕过散射和衍射极限。

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