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Stærkt Løst Intravital Striped-belysning mikroskopi af kimcentre
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1Biophysical Analytics, German Rheumatism Research Center,Leibniz Institute, 2Microscopy Core Facility,Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center,Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging,Charité – University of Medicine
Chapters
- 00:05Title
- 01:26Multi-beam Setup for Striped-illumination TPLSM
- 06:07Results: Highly-resolved Intravital Striped-illumination Images
- 09:05Conclusion
Høj opløsning intravital billeddannelse med forbedret kontrast op til 120 um dybde i lymfeknuder voksne mus opnås ved rumligt at modulere excitation mønster af en multifokal to-foton mikroskop. I 100 um dybde målt vi beslutninger af 487 nm (laterale) og 551 nm (aksialt), derved omgå spredning og diffraktion grænser.
