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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
어린 싹 센터의 높은 해결의 intravital 스트라이프 - 조명 현미경
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어린 싹 센터의 높은 해결의 intravital 스트라이프 - 조명 현미경

Article DOI: 10.3791/51135
April 9th, 2014 •
*1,2, *3, 4, 2, *3,5, *1
1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
* These authors contributed equally

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April 9th, 2014

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성체 마우스의 림프절에서 120 ㎛의 깊이까지의 향상된 콘트라스트와 높은 해상도의 intravital 촬상 공간적 다중 초점 이광자 현미경의 여기 패턴을 변조함으로써 달성된다. 100 μm의 깊이에서 우리는 따라서 산란 및 회절 한계를 우회, 487 ㎚의 해상도 (측면) 및 551 nm의 (축 방향)을 측정 하였다.

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