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Journal / Immunology and Infection / Meget Løst Intravital Striped-belysning…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Meget Løst Intravital Striped-belysning Mikroskopi av Germinal Centers
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Meget Løst Intravital Striped-belysning Mikroskopi av Germinal Centers

Article DOI: 10.3791/51135-v • 10:07 min • April 9th, 2014
April 9th, 2014 •
*1,2, *3, 4, 2, *3,5, *1
1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine
* These authors contributed equally

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Høyoppløselig intravital avbildning med forbedret kontrast til 120 mikrometer dybde i lymfeknuter hos voksne mus oppnås ved romlig modulerende magnetisering mønster av en multi-fokal to-foton mikroskop. I 100 mikrometer dybde målte vi en oppløsning på 487 nm (laterale) og 551 nm (aksial), og dermed omgå spredning og diffraksjon grenser.

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Immunologi to-foton laser scanning mikroskopi dype vev intra bildebehandling germinal sentrum lymfeknute høy oppløsning forbedret kontrast
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