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JoVE Journal Neuroscience
Differential Mærkning af Celle-overflade og internaliseres Proteiner efter Antibody Fodring Live dyrkede neuroner
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Differential Mærkning af Celle-overflade og internaliseres Proteiner efter Antibody Fodring Live dyrkede neuroner

Article DOI: 10.3791/51139-v • 11:56 min • February 12th, 2014
February 12th, 2014 •
*1, *1, 1, 2, 1,3
1Department of Anatomy & Neuroscience, The University of Melbourne, 2Department of Neurobiology, Duke University Medical Center, 3Florey Institute of Neuroscience & Mental Health, The University of Melbourne
* These authors contributed equally

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Vi beskriver en fremgangsmåde til at mærke proteiner på overfladen af ​​levende neuroner under anvendelse af et specifikt polyklonalt antistof mod ekstracellulære epitoper. Protein bundet af antistof på celleoverfladen og derefter internaliseres via endocytose kan skelnes fra protein forbliver på eller handles til overfladen under inkubationen.

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Neurovidenskab to-farvet fluorescens immuncytokemi menneskehandel endocytose genbrug endosome neuroner
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