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JoVE Journal
Biology

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最快在西镇:对经典蛋白质印迹分析现代风味
 
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最快在西镇:对经典蛋白质印迹分析现代风味

Article DOI: 10.3791/51149-v 11:43 min February 5th, 2014
February 5th, 2014

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

该协议探索的最新进展进行免疫印迹分析。这些新颖的改进采用的Bis-Tris凝胶系统用35分钟电泳运行时,一个7分钟的干印迹转移系统和红外荧光蛋白的检测和成像,其产生更高的分辨率,质量,灵敏度和西数据的改进的准确度。

Tags

基本协议,发行84,Western blot法的Bis-Tris,电泳,干燥的印迹,蛋白转移,红外,荧光,量化,抗体,蛋白质
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