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Rapid Synthese en Screening van chemisch geactiveerde transcriptie Factoren met GFP gebaseerde Reporters

Article DOI: 10.3791/51153-v • 09:22 min • November 26th, 2013
November 26th, 2013 •
*1,3, *1, 1,2, 1
1The Lewis-Sigler Institute for Integrative Genomics, Princeton University, 2Department of Molecular Biology, Princeton University, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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Dit protocol beschrijft een experimentele procedure voor de snelle bouw van kunstmatige transcriptiefactoren (ATF) met verwante GFP verslaggevers en kwantificering van de ATFs mogelijkheid om GFP-expressie te stimuleren via flowcytometrie.

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Genetica transcriptie transcriptiefactoren kunstmatige transcriptiefactoren zink vingers Zif268 synthetische biologie
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