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Analyse van de translatie-initiatie tijdens stress Voorwaarden door polysome Profiling
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Analyse van de translatie-initiatie tijdens stress Voorwaarden door polysome Profiling

Article DOI: 10.3791/51164-v • 10:59 min • May 19th, 2014
May 19th, 2014 •
*1,2, *1,2, 1,2
1Department of Molecular Biology, Medical Biochemistry, and Pathology, Faculty of Medicine, Laval University, 2CHU de Quebec Research Center
* These authors contributed equally

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Hier beschrijven we een werkwijze veranderingen in de initiatie van mRNA translatie van eukaryote cellen in reactie stresssituaties analyseren. Deze methode is gebaseerd op de snelheid scheiding op sucrosegradiënten vertalen ribosomen van niet-translating ribosomen.

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Cellular Biology translatie-initiatie polysome profiel sucrosegradiënt eiwit-en RNA-isolatie stress-condities
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