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Cerebrospinalvæsken MikroRNA Profilering Bruke Kvantitativ Real Time PCR

Article DOI: 10.3791/51172-v • 09:26 min • January 22nd, 2014
January 22nd, 2014 •
1, 2, 1, 1
1Medical School and Stanley S. Scott Cancer Center, LSU Health Sciences Center, 2Department of Biomedical, Surgery and Dental Sciences, University of Milan

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Vi beskriver en protokoll av sanntids PCR å profilere microRNAs i cerebrospinalvæsken (CSF). Med unntak av RNA-ekstraksjonsmetoder, kan fremgangsmåten bli utvidet til RNA ekstrahert fra andre kroppsvæsker, dyrkede celler, eller vevsprøver.

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Medisin microRNAs biomarkører miRNA profilering qPCR spinalvæske RNA DNA
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