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JoVE Journal Biology
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FtsZ Polymerization Assays: Simple Protocols and Considerations
Journal (Biology)
FtsZ Polymerization Assays: Simple Protocols and Considerations
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
Journal (Biology)
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
 
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Article DOI: 10.3791/51178-v • 07:37 min • February 13th, 2014
February 13th, 2014 •
1, 2, 2, 1
1Microbial Biotechnology, Institute Biology Leiden, Leiden University, 2Microbiology, Kluyver Centre for Genomics of Industrial Fermentation, Utrecht University

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Væske dyrkes Streptomyces kulturer er kjennetegnet ved mycelie pellets som er heterogene i størrelse. Vi her beskrive en metode for å analysere og sortere slike pellets i en high-throughput måte. Disse pellets kan anvendes for videre analyser, som vil gi ledninger for å forstå og kontrollere veksten heterogenitet.

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Mikrobiologi , Flowcytometri pellets morfologi fluorescens Copas bioteknologi high-throughput analyser sortering heterogenitet
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