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JoVE Journal Chemistry
Isolering og forberedelse af bakteriecellevægge til kompositorisk analyse af ultraperformet væskekromatografi
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Isolering og forberedelse af bakteriecellevægge til kompositorisk analyse af ultraperformet væskekromatografi

Article DOI: 10.3791/51183-v • 11:18 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 1,4
1Department of Bioengineering, Stanford University, 2Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, Umeå University, 3Campus de Cantoblanco, Universidad Autonoma de Madrid, 4Department of Microbiology and Immunology, Stanford University School of Medicine

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Bakteriecellevæggen består af peptidoglycan, et makromolekylært netværk af sukkerstrenge, der krydses af peptider. Ultra Performance Liquid Chromatography giver høj opløsning og gennemløb for nye opdagelser af peptidoglycan sammensætning. Vi præsenterer en procedure for isolering af cellevægge (sacculi) og deres efterfølgende forberedelse til analyse via UPLC.

Tags

Kemi Problem 83 peptidoglycan bakteriecellevæg ultraperformet væskekromatografi højtydende væskekromatografi celleform morfogenese
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