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Bestämma isbindande plan för frostskyddsproteiner genom fluorescensbaserad isplanstillhörighet
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Bestämma isbindande plan för frostskyddsproteiner genom fluorescensbaserad isplanstillhörighet

Article DOI: 10.3791/51185-v • 08:46 min • January 15th, 2014
January 15th, 2014 •
1, 2, 3, 3, 4, 1
1Department of Biomedical and Molecular Sciences, Queen's University, 2National Institute of Neurological Disorders and Stroke, Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem

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Frostskyddsmedel (AFPs) binder till specifika isplan för att förhindra eller bromsa istillväxten. Fluorescensbaserad isplanstillhörighet (FIPA) analys är en modifiering av den ursprungliga is-etsningsmetoden för bestämning av AFP-bundna isplan. AFPs är fluorescerande märkta, införlivade i makroskopiska enstaka iskristaller och visualiserade under UV-ljus.

Tags

Kemi Nummer 83 Material Biovetenskap Optik frostskyddsmedel Is adsorption Fluorescerande märkning Isgitterplan isbindande proteiner Eniskristall
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