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Journal / Immunology and Infection / Isolatie van Double Negative αβ T-cellen uit de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolatie van Double Negative αβ T-cellen uit de nier
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Isolatie van Double Negative αβ T-cellen uit de nier

Article DOI: 10.3791/51192-v • 06:56 min • May 16th, 2014
May 16th, 2014 •
1, 2, 2, 1
1Department of Pathology, Johns Hopkins University School of Medicine, 2Department of Medicine, Johns Hopkins University School of Medicine

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DNabT cellen zijn zeldzaam onder perifere T-cellen; maar ze zijn er in overvloed in bepaalde niet-lymfoïde weefsels. Moeilijkheid van het isoleren van DN T-cellen van niet-lymfeweefsel belemmert hun functionele analyse Ondanks toenemende erkend pathofysiologische betekenis. We beschrijven een nieuw protocol voor isolatie van hooggezuiverde DN T-cellen van muizen nier.

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Immunologie Double Negative (DN) αβ T-cellen CD45 + T-cel isolatie nier-lymfocyten niet-lymfoïde-weefsels T-cellen zuivering ischemiereperfusieletsel acute nierschade Tissue Resident lymfocyten lymfoproliferatieve aandoeningen erythematosus Lupus
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