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Journal / Immunology and Infection / Böbrek gelen Çift Negatif αβ T Hücreleri…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Böbrek gelen Çift Negatif αβ T Hücreleri İzolasyon
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Böbrek gelen Çift Negatif αβ T Hücreleri İzolasyon

Article DOI: 10.3791/51192-v • 06:56 min • May 16th, 2014
May 16th, 2014 •
1, 2, 2, 1
1Department of Pathology, Johns Hopkins University School of Medicine, 2Department of Medicine, Johns Hopkins University School of Medicine

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DNabT hücreleri periferal T-hücreleri arasında nadirdir; Bununla birlikte, belli olmayan lenfoid dokuda bol miktarda bulunmaktadır. Non-lenfoid doku DN T hücrelerini izole Zorluk patofizyolojik önemini kabul kapasitelerine rağmen fonksiyonel analizini engellemektedir. Bu murin böbreğinden yüksek derecede arıtılmış DN T hücrelerinin izole edilmesi için yeni bir protokol açıklar.

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İmmünoloji Sayı 87 Çift Negatif (DN) αβ T hücreleri CD45 + T hücre izolasyonu böbrek lenfositler non-lenfoid-dokular T hücreleri arıtma iskemi reperfüzyon hasarı akut böbrek hasarı Doku Resident Lenfosit Lenfoproliferatif Bozuklukları Eritematoz Lupus
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