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Isolering av Blood-fartøy-avledet multipotent Forstadier fra Menneskelig Skeletal Muscle
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Isolering av Blood-fartøy-avledet multipotent Forstadier fra Menneskelig Skeletal Muscle

Article DOI: 10.3791/51195-v • 10:52 min • August 21st, 2014
August 21st, 2014 •
1, 2,3, 4, 5, 6, 7,8, 9
1Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Pittsburgh, 3Nazarbayev University Research and Innovation System, Nazarbayev University, 4Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 5Department of Cell Biology, Erasmus MC Stem Cell Institute, 6OHSU Center for Regenerative Medicine, Oregon Health & Science University, 7Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh, 8David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 9Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh

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Blodårene i menneskelige skjelettmuskulatur havne flere multi-avstamning forløper populasjoner som er ideelle for regenerativ applikasjoner. Denne isolasjonsmetoden tillater samtidig rensing av tre multipotente forløper cellepopulasjoner henholdsvis fra tre strukturelle lag av blodkar: myogeniske endotelceller fra intima, pericytes fra media, og adventitial celler fra adventitia.

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Cellular Biology Blood Vessel; Pericyte; Adventitial Cell; Myogenic endotelcelle; Multipotent Precursor
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