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Effektiv Produktion og oprensning af rekombinant murin Kindlin-3 fra Insect Celler til Biofysiske Studies
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Effektiv Produktion og oprensning af rekombinant murin Kindlin-3 fra Insect Celler til Biofysiske Studies

Article DOI: 10.3791/51206-v • 13:52 min • March 19th, 2014
March 19th, 2014 •
1, 1
1Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford

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Kindlins er grundlæggende for celle adhæsion gennem integriner men undersøgelser af dem har været hæmmet af vanskeligheden ved at udtrykke dem rekombinant i bakterielle værter. Vi beskriver her metoder til deres effektive produktion i baculovirus-inficerede insektceller.

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Virology Heterolog proteinekspression insektceller proteinoprensning kindlin celleadhæsion
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