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13:52 min
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March 19, 2014
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Summary
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Kindlins sono fondamentali per l'adesione cellulare tramite integrine ma gli studi di loro sono stati ostacolati dalla difficoltà incontrata ad esprimere loro ricombinante in ospiti batterici. Descriviamo qui metodi per la loro produzione efficiente in cellule di insetto baculovirus-infette.
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Cite this Article
Yates, L. A., Gilbert, R. J. C. Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies. J. Vis. Exp. (85), e51206, doi:10.3791/51206 (2014).
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