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Journal / Immunology and Infection / Avaliando a inata Sensing de HIV-1 CD4 Infected
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Avaliando a inata Sensing de HIV-1 CD4 Infected
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Avaliando a inata Sensing de HIV-1 CD4 Infected

Article DOI: 10.3791/51207-v • 08:11 min • September 1st, 2015
September 1st, 2015 •
1, 1, 1,2
1Laboratory of Human Retrovirology, Institut de Recherches Cliniques de Montréal (IRCM), 2Department of Microbiology, Infectiology, and Immunology, Université de Montréal

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Ao contrário de partículas de HIV-1 livre de células, as células T CD4 + infectadas são eficazmente detectada pelas células dendríticas plasmocitoides (PDCs). Este manuscrito descreve um método em que as células mononucleares do sangue periférico (PBMCs) ou pDCs isoladas são co-cultivadas com o HIV-1 de células T infectadas para avaliar a detecção inata por pDCs como avaliada por libertação de IFN tipo I.

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Immunology edição 103 Infecções por HIV Immunity inata HIV-1 pDC sensoriamento inata tipo I IFN
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