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JoVE Journal Neuroscience
Vurdering af cellecyklusprogression af neurale stam-og stamcelle i Mouse hjernens udvikling efter Genotoksisk Stress
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Vurdering af cellecyklusprogression af neurale stam-og stamcelle i Mouse hjernens udvikling efter Genotoksisk Stress

Article DOI: 10.3791/51209-v • 09:51 min • May 7th, 2014
May 7th, 2014 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4
1Laboratoire de Radiopathologie, CEA DSV iRCM SCSR, 2INSERM, U967, 3Université Paris Diderot, Sorbonne Paris Cité, 4Université Paris Sud, UMR 967

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Administration af to analoger af thymidin, Edu og BrdU, i gravide mus tillader analyse af cellecyklus progression i neurale og stamceller i embryonale mus hjernen. Denne metode er nyttig til at bestemme virkningerne af genotoksisk stress, herunder ioniserende stråling under hjernens udvikling.

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Neuroscience Edu BrdU, neurale stamceller og stamceller cellecyklus embryonale cortex immunfarvning cellecykluskontrolpunkter apoptose genotoksisk stress embronic mus hjerne
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