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JoVE Journal Bioengineering
Mikrofluid Platform for Måling neutrofilkemotaksi fra uforarbejdede fuldblod
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Mikrofluid Platform for Måling neutrofilkemotaksi fra uforarbejdede fuldblod

Article DOI: 10.3791/51215-v • 10:13 min • June 3rd, 2014
June 3rd, 2014 •
*1,2,3, *1,2,3, 1, 1, 1,4, 1,2,3
1The BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital, 2Harvard Medical School, 3Shriners Burns Hospital, 4Harvard University School of Engineering and Applied Sciences
* These authors contributed equally

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Denne protokol beskriver en analyse designet til at måle menneskelige neutrofilkemotaksi fra en dråbe fuldblod med robust reproducerbarhed. Denne fremgangsmåde omgår behovet for neutrofil separation og kræver kun et par minutter af assay forberedelsestid. Mikrofluid chip muliggør gentagen måling af neutrofilkemotaksi over tid hos spædbørn eller små pattedyr, hvor prøvevolumen er begrænset.

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Bioteknik chemotaxis neutrofile fuldblodsanalysen mikrofluid enhed kemoattraktant migration inflammation
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