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JoVE Journal Neuroscience
En cGMP-gældende Expansion Metode til aggregater af humane neurale Stem og stamceller arvet fra pluripotente stamceller eller fostrets hjerne Tissue
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En cGMP-gældende Expansion Metode til aggregater af humane neurale Stem og stamceller arvet fra pluripotente stamceller eller fostrets hjerne Tissue

Article DOI: 10.3791/51219-v • 09:37 min • June 15th, 2014
June 15th, 2014 •
1, 1, 1
1Regenerative Medicine Institute, Cedars-Sinai Medical Center

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Denne protokol beskriver en ny mekanisk sønderdeling, der tillader udvidelse af sfæriske neurale stamceller og stamcelle aggregater uden dissociation til en enkelt cellesuspension. Fastholdelse af celle / celle kontakt giver mulighed for hurtig og stabil vækst i over 40 passager.

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Neuroscience neural progenitorcelle neural precursorcelle neurale stamceller passage neurosfære hakke stamceller neurovidenskab suspension kultur god fremstillingspraksis GMP
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