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JoVE Journal Neuroscience
En cGMP-relevant Expansion Metode for Aggregater of Human Neural Stem og stamceller avledet fra pluripotent stamceller eller Fetal hjernevev
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En cGMP-relevant Expansion Metode for Aggregater of Human Neural Stem og stamceller avledet fra pluripotent stamceller eller Fetal hjernevev

Article DOI: 10.3791/51219-v • 09:37 min • June 15th, 2014
June 15th, 2014 •
1, 1, 1
1Regenerative Medicine Institute, Cedars-Sinai Medical Center

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Denne protokollen beskriver en ny mekanisk hakking metode som tillater utvidelse av sfæriske nevrale stamceller og progenitor-celleaggregater uten dissosiasjon til en enkelt cellesuspensjon. Opprettholde celle / celle kontakt tillater rask og stabil vekst i over 40 passasjer.

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Neuroscience nevrale stamceller nevrale forløper celle nevrale stamceller aging neurosphere hakking stamcelle nevrovitenskap suspensjon kultur god tilvirkningspraksis GMP
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