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Journal / Immunology and Infection / Hochdurchsatz-Screening für…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Hochdurchsatz-Screening für Breitspektrum-Chemische Inhibitoren der RNA-Viren
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Hochdurchsatz-Screening für Breitspektrum-Chemische Inhibitoren der RNA-Viren

Article DOI: 10.3791/51222-v • 11:34 min • May 5th, 2014
May 5th, 2014 •
1, 2, 2, 1, 3, 1, 1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur

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In-vitro-Assays zur Messung der Virusreplikation wurde durch die Entwicklung von rekombinanten RNA-Viren Luciferase oder andere Enzyme, die Biolumineszenz exprimieren verbessert. Hier werden wir ausführlich ein Hochdurchsatz-Screening-Pipeline, die solche rekombinante Stämme von Masern-und Chikungunya-Viren, um Breitspektrum antiviralen Medikamenten aus chemischen Bibliotheken zu isolieren verbindet.

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Immunologie Virusinfektionen Hochdurchsatz-Screening-Assays Breitspektrum antiviralen Medikamenten Chikungunya-Virus Masern-Virus Luciferase-Reporter chemischen Bibliotheken
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