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Immunology and Infection

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JoVE Journal Immunology and Infection
Triagem de alta capacidade para amplo espectro químico Inibidores de Vírus RNA
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Triagem de alta capacidade para amplo espectro químico Inibidores de Vírus RNA

Article DOI: 10.3791/51222-v • 11:34 min • May 5th, 2014
May 5th, 2014 •
1, 2, 2, 1, 3, 1, 1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur

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Os ensaios in vitro para medir a replicação do vírus tem sido melhorada pelo desenvolvimento de vírus de RNA recombinantes que expressam luciferase ou outras enzimas capazes de bioluminescência. Aqui detalhamos uma triagem gasoduto de alto rendimento, que combina essas linhagens recombinantes de sarampo e vírus chikungunya para isolar os antivirais de amplo espectro de bibliotecas químicas.

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Imunologia Edição 87 infecções virais de alto rendimento testes de rastreio antivirais de amplo espectro vírus chikungunya vírus do sarampo luciferase repórter bibliotecas químicas
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