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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput screening per largo spettro chimico inibitori di RNA virus
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High-throughput screening per largo spettro chimico inibitori di RNA virus

Article DOI: 10.3791/51222-v • 11:34 min • May 5th, 2014
May 5th, 2014 •
1, 2, 2, 1, 3, 1, 1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur

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Saggi in vitro per misurare la replicazione del virus sono state notevolmente migliorate dallo sviluppo di virus a RNA ricombinanti esprimenti luciferasi o altri enzimi in grado di bioluminescenza. Qui i dettagli di una pipeline screening ad alto rendimento che unisce tali ceppi ricombinanti di morbillo e virus chikungunya per isolare antivirali ad ampio spettro di librerie chimiche.

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Immunologia a infezioni virali ad alto throughput saggi di screening antivirali ad ampio spettro virus chikungunya virus del morbillo reporter della luciferasi librerie chimiche
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