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Journal / Neuroscience / Um rato, duas culturas: isolamento e cultivo de…
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Neuroscience

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JoVE Journal Neuroscience
Um rato, duas culturas
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Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
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Journal (Neuroscience)
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Um rato, duas culturas: isolamento e cultivo de células-tronco neurais Adulto de as duas zonas neurogênica Individual Ratos

Article DOI: 10.3791/51225-v • 09:52 min • February 25th, 2014
February 25th, 2014 •
1, 1,2
1Center for Regenerative Therapies Dresden, Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden

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Aqui, descrevemos um protocolo detalhado para a produção simultânea de culturas de células precursoras neurais, quer como monocamadas aderentes ou neuroesferas, a partir da zona subventricular e giro dentado de ratinhos adultos individuais.

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Neurociência Edição 84 célula precursora neurosphere monocamada aderente zona subventricular giro denteado rato adulto
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