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Journal / Neuroscience / Brain Slice Biotinylering: An
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Brain Slice Biotinylering: An

Article DOI: 10.3791/51240-v • 06:18 min • April 3rd, 2014
April 3rd, 2014 •
1, 1, 2
1Program in Neuroscience, Graduate School of Biomedical Sciences, University of Massachusetts Medical School, 2Brudnick Neuropsychiatric Research Institute, Department of Psychiatry, University of Massachusetts Medical School

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Neuronal membran människohandel kontrollerar dynamiskt plasmamembranprotein tillgänglighet och kraftigt påverkan neurotransmission. Hittills har det varit en utmaning att mäta neuronal endocytic handel med vuxna nervceller. Här beskriver vi en mycket effektiv, kvantitativ metod för att mäta snabba förändringar i ytprotein uttryck ex vivo i akuta hjärnsnitt.

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Neurovetenskap Trafficking endocytos internalisering biotinylering hjärna neuroner transportör proteinkinas C
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