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Elicoidale Organization of Blood fattore VIII della coagulazione su Lipid nanotubi
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Elicoidale Organization of Blood fattore VIII della coagulazione su Lipid nanotubi

Article DOI: 10.3791/51254-v • 12:24 min • June 3rd, 2014
June 3rd, 2014 •
*1, *1, 2,3, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
* These authors contributed equally

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Vi presentiamo una combinazione di crio-microscopia elettronica, lipidi nanotecnologie, e analisi della struttura applicata per risolvere la struttura di membrana delle due forme di FVIII altamente omologhi: umane e suine. La metodologia sviluppata nel nostro laboratorio per organizzare elicoidale le due forme contenenti Fattore VIII ricombinante funzionali su nanotubi di lipidi carichi negativamente (LNT) è descritto.

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Bioingegneria crio-microscopia elettronica i nanotubi di lipidi montaggio elicoidale organizzazione legata alla membrana fattore VIII della coagulazione
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