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JoVE Journal Bioengineering
Helical Organisation av koagulationsfaktor VIII på Lipid Nanorör
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Helical Organisation av koagulationsfaktor VIII på Lipid Nanorör

Article DOI: 10.3791/51254-v • 12:24 min • June 3rd, 2014
June 3rd, 2014 •
*1, *1, 2,3, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
* These authors contributed equally

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Vi presenterar en kombination av Cryo-elektronmikroskopi, lipid nanoteknik, och strukturanalys tillämpas för att lösa membranbundna strukturen av två mycket homologa FVIII former: mänskliga och svin. Den metod som utvecklats i vårt laboratorium för att spiral organisera de två funktionella rekombinanta FVIII formulär på negativt laddade lipid nanorör (LNT) beskrivs.

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Bioengineering Cryo-elektronmikroskopi Lipid nanorör Helical montering membranbunden organisation koagulationsfaktor VIII
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