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JoVE Journal Bioengineering
Tandhjulsgear Organization of Blood koagulationsfaktor VIII på Lipid Nanorør
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Tandhjulsgear Organization of Blood koagulationsfaktor VIII på Lipid Nanorør

Article DOI: 10.3791/51254-v • 12:24 min • June 3rd, 2014
June 3rd, 2014 •
*1, *1, 2,3, 1, 1,3
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 2Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, 3Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch
* These authors contributed equally

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Vi præsenterer en kombination af Cryo-elektronmikroskopi, lipid nanoteknologi og struktur analyse anvendes for at løse den membranbundne struktur med to meget homologe FVIII former: menneskelige og svin. Den metode, udviklet i vores laboratorium for at skruelinieform organisere de to funktionelle rekombinante FVIII formularer på negativt ladede lipid-nanorør (LNT) er beskrevet.

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Bioengineering Cryo-elektronmikroskopi lipidnanorør Helical samling Membranbundet organisation koagulationsfaktor VIII
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