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Neuroscience

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JoVE Journal Neuroscience
In vivo Interrogatorio del Sistema Nervoso Centrale Translatome da Polyribosome Frazionamento
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In vivo Interrogatorio del Sistema Nervoso Centrale Translatome da Polyribosome Frazionamento

Article DOI: 10.3791/51255-v • 09:13 min • April 30th, 2014
April 30th, 2014 •
1, 1, 1, 1
1Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)

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Questo protocollo illustra le modifiche essenziali della polyribosome frazionamento, al fine di studiare la translatome di vivo campioni SNC in. Permette valutazione globale della regolamentazione traduzione e trascrizione attraverso l'isolamento e il confronto di RNA totale di ribosoma frazioni di RNA legati.

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Neuroscienze Numero 86 sistema nervoso centrale SNC traduzione polyribosome frazionamento RNA cervello midollo spinale microarray sequenziamento di prossima generazione pendenza translatome
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