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JoVE Journal Neuroscience
Imaging dendritiske udløbere af Rat Primær hippocampusneuroner hjælp Structured Illumination Mikroskopi
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Imaging dendritiske udløbere af Rat Primær hippocampusneuroner hjælp Structured Illumination Mikroskopi

Article DOI: 10.3791/51276-v • 14:11 min • May 4th, 2014
May 4th, 2014 •
*1, *2, 1, 2, 1, 1, 1, 2, 1
1Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam
* These authors contributed equally

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Denne artikel beskriver en arbejdsgruppe protokol til billede dendritiske Torner fra hippocampus neuroner in vitro ved hjælp af Structured Illumination Microscopy (SIM). Super-opløsning mikroskopi hjælp SIM giver billedopløsning betydeligt over den lys diffraktion grænse i alle tre rumlige dimensioner, så billeder af de enkelte dendritiske Torner med forbedret detalje.

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Neuroscience dendritiske Spine mikroskopi Confocal Fluorescens Neurosciences hippocampus den primære neuron super opløsning mikroskopi struktureret belysning mikroskopi (SIM) neurovidenskab Dendrit
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