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Immunology and Infection

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JoVE Journal Immunology and Infection
Ativação e Mensuração de NLRP3 inflammasome Atividade Usando IL-1β em células dendríticas humanas Monocyte derivados
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Ativação e Mensuração de NLRP3 inflammasome Atividade Usando IL-1β em células dendríticas humanas Monocyte derivados

Article DOI: 10.3791/51284-v • 09:04 min • May 22nd, 2014
May 22nd, 2014 •
1, 2, 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

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As células dendríticas (CDs) segregam IL-1β em resposta ao reconhecimento de purina TLR8 sintético, R848, seguido por activação inflammasome NLRP3 com nigericina, por conseguinte, a IL-1β pode ser utilizado para medir a actividade inflammasome NLRP3. Coloração intracelular de citocinas, immunoblotting e ELISA são usados ​​para medir com precisão NLRP3 inflammasome priming e ativação via expressão de IL-1β.

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Imunologia Edição 87 NLRP3 inflammasome IL-1beta interleucina-1 beta dendríticas células nigericina Toll-like receptor 8 TLR8 R848 células dendríticas derivadas de monócitos
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