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Immunology and Infection

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JoVE Journal Immunology and Infection
Aktivering og måling af NLRP3 Inflammasome Aktivitet hjælp af IL-1β i Human monocytafledte dendritiske celler
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Aktivering og måling af NLRP3 Inflammasome Aktivitet hjælp af IL-1β i Human monocytafledte dendritiske celler

Article DOI: 10.3791/51284-v • 09:04 min • May 22nd, 2014
May 22nd, 2014 •
1, 2, 3
1Department of Pathology, New York University School of Medicine, 2Division of Infectious Diseases, Department of Medicine, Mount Sinai Medical Center, 3Division of Hematology and Oncology, Hess Center for Science and Medicine, Mount Sinai Medical Center

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Dendritiske celler (DC'er) udskiller IL-1β som reaktion på TLR8 anerkendelse af syntetisk purin, R848, efterfulgt af NLRP3 inflammasome aktivering med nigericin derfor IL-1β kan anvendes til at måle NLRP3 inflammasome aktivitet. Intracellulær cytokin farvning, immunoblotting og ELISA anvendes til præcist at måle NLRP3 inflammasome priming og aktivering via IL-1β udtryk.

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Immunologi NLRP3 inflammasome IL-1 beta interleukin-1 beta dendritiske Cell nigericin Toll-like receptor 8 TLR8 R848 monocytafledt dendritiske celler
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