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Analysering proteasomalaktivitet Nedbrydning i en celle-fri system i planter
Title Cell Encapsulation by Droplets
Journal (Biology)
Title Cell Encapsulation by Droplets
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
Journal (Biology)
Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
 
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Analysering proteasomalaktivitet Nedbrydning i en celle-fri system i planter

Article DOI: 10.3791/51293-v • 07:43 min • March 26th, 2014
March 26th, 2014 •
1, 1, 1
1Department of Biochemistry and Cell Biology, Stony Brook University, State University of New York

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Målrettet protein nedbrydning udgør en stor reguleringsmekanisme for celle funktion. Det sker via en konserveret ubiquitinproteasomvejen, som lægger multiubiquitinkæder til målproteinet som derefter tjene som molekylære "tags" for 26S proteasom. Her beskriver vi en enkel og pålidelig cellefrit assay for proteasomalaktivitet nedbrydning af proteiner.

Tags

Biokemi Ubiquitin / proteasome-system 26S proteasomet protein nedbrydning proteasominhibitor Western blotting plante genetisk transformation
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