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Partikül izleme Microrheology kullanarak 3D Tümör Modelleri Ekstrasellüler Matriks Rijitliğinin Boyuna Ölçümü
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology
DOI:

11:11 min

June 10, 2014

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Chapters

  • 00:05Title
  • 01:25Culturing Tumor Spheroids
  • 02:54Preparing 3D Tumor Spheroids Embedded in ECM-containing Tracer Probes
  • 05:33Construction of Sample Point Grid and Video Acquisition at Each Point
  • 07:34Video Data Analysis for Computation of Rheological Properties at Each Spatial Position
  • 09:10Results: Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models
  • 10:26Conclusion

Summary

Automatic Translation

Parçacık izleme microrheology olmayan yıkıcı ölçmek ve 3 boyutlu uzamsal tümör modellerinde hücre dışı matris mekanik özelliklerinde değişiklikler eşlemek için kullanılabilir.

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