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JoVE Journal Neuroscience
Anvendelse af en Caspase Multiplexing assay til bestemmelse af apoptose i en Hypothalamus Cell Model
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Anvendelse af en Caspase Multiplexing assay til bestemmelse af apoptose i en Hypothalamus Cell Model

Article DOI: 10.3791/51305-v • 08:27 min • April 16th, 2014
April 16th, 2014 •
1,2, 1,2, 3, 1,2,4, 1,2, 1,2
1Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition, University of Minnesota, 3Department of Integrative Biology and Physiology, University of Minnesota, 4Department of Medicine, University of Minnesota Medical School, University of Minnesota

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Multiplex analyser kan give gavnlig information for basale cellulære mekanismer og eliminere spild af reagenser og unødvendige gentagne eksperimenter. Vi beskriver her en multiplex caspase-3/7 aktivitet assay anvendelse af fluorescerende-og luminescerende-baserede metoder, for at bestemme cellelevedygtighed i en in vitro hypothalamus model efter oxidativ udfordring med palmitinsyre.

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Neuroscience apoptose fedme caspase resazurin DEVD palmitinsyre hypothalamus model
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