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JoVE Journal Neuroscience
Användning av en kaspas Multiplexing Assay att Bestäm Apoptos i en Hypotalamus Cell Model
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Användning av en kaspas Multiplexing Assay att Bestäm Apoptos i en Hypotalamus Cell Model

Article DOI: 10.3791/51305-v • 08:27 min • April 16th, 2014
April 16th, 2014 •
1,2, 1,2, 3, 1,2,4, 1,2, 1,2
1Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition, University of Minnesota, 3Department of Integrative Biology and Physiology, University of Minnesota, 4Department of Medicine, University of Minnesota Medical School, University of Minnesota

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Multiplex-analyser kan ge bra information om grundläggande cellulära mekanismer och eliminera slöseri med reagenser och onödiga upprepade experiment. Vi beskriver här en multiplex-kaspas-3/7 aktivitetsanalys, genom att använda fluorescens och luminiscerande baserade metoder, för att bestämma cellviabilitet i en in vitro hypotalamisk modell följande oxidativa utmaning med palmitinsyra.

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Neuroscience apoptos fetma kaspas resazurin DEVD palmitinsyra hypotalamisk modell
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