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JoVE Journal Neuroscience
L'uso di una caspasi Multiplexing test per determinare apoptosi in un modello cellulare ipotalamica
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L'uso di una caspasi Multiplexing test per determinare apoptosi in un modello cellulare ipotalamica

Article DOI: 10.3791/51305-v • 08:27 min • April 16th, 2014
April 16th, 2014 •
1,2, 1,2, 3, 1,2,4, 1,2, 1,2
1Department of Veterans Affairs, Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition, University of Minnesota, 3Department of Integrative Biology and Physiology, University of Minnesota, 4Department of Medicine, University of Minnesota Medical School, University of Minnesota

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Saggi Multiplex possono fornire informazioni utile per i meccanismi cellulari di base ed eliminare gli sprechi di reagenti ed esperimenti ripetitivi inutili. Descriviamo qui un multiplex caspasi-3/7 saggio di attività, utilizzando metodi fluorescenti e si basano luminescenti-, per determinare la vitalità cellulare in un modello in vitro ipotalamico seguente sfida ossidativa con acido palmitico.

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Neuroscienze Numero 86 l'apoptosi l'obesità la caspasi resazurina DEVD acido palmitico modello ipotalamico
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