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JoVE Journal
Immunology and Infection

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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
 
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Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Article doi: 10.3791/51306
November 3rd, 2014

Summary November 3rd, 2014

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In this protocol, we describe methods to efficiently transfect murine macrophage cell lines with siRNAs using the Amaxa Nucleofector 96-well Shuttle System, stimulate the macrophages with lipopolysaccharide, and monitor the effects on inflammatory cytokine production.

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