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Análisis de las reacciones de procesamiento del ARN que utilizan teléfonos Free Systems
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Análisis de las reacciones de procesamiento del ARN que utilizan teléfonos Free Systems: 3 'End división de pre-mRNA Sustratos

Article DOI: 10.3791/51309-v • 09:16 min • May 3rd, 2014
May 3rd, 2014 •
1, 1, 2, 1
1Department of Infectious Diseases, The Scripps Research Institute, 2Department of Chemistry, City College of New York

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ARN polimerasa II sintetiza un precursor de ARN que se extiende más allá del extremo 3 'del ARNm maduro. El final de la ARN maduro se genera cotranscriptionally, en un sitio de dictado por las secuencias de ARN, a través de la actividad de endonucleasa del complejo de escisión. Aquí, detalle el método para estudiar las reacciones de escisión in vitro.

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Complejo de Enfermedades Infecciosas Escote Polyadenylation el procesamiento del mRNA extractos nucleares 3 'Procesamiento
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